What is SYBR Green Master Mix?

HomeWhat is SYBR Green Master Mix?
What is SYBR Green Master Mix?

GoTaq® DNA Polymerase is a proprietary formulation of Taq polymerase that gives robust amplification equal to and, in some cases, superior to that of standard Taq.

Q. What does GoTaq Master Mix contain?

GoTaq® Green Master Mix is a premixed ready-to-use solution containing bacterially derived Taq DNA polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR.

Q. What is GoTaq?

GoTaq® DNA Polymerase is a proprietary formulation of Taq polymerase that gives robust amplification equal to and, in some cases, superior to that of standard Taq.

Description. Green Taq DNA Polymerase is designed to increase the stability of the Taq enzyme for more convenient transport and applications. The polymerase (1000 U) is designed for 400 rxns if 2.5 U are used per 50 ul PCR reaction.

Q. Why is GoTaq master mix dyed?

GoTaq® Green Master Mix also includes two dyes (blue and yellow) that allow monitoring of progress during electrophoresis. The blue dye comigrates at the same rate as 3–5kb DNA fragments in a 1% agarose gel. The yellow dye migrates ahead of primers (<50bp).

Q. What is the purpose of mgcl2 in PCR?

Posted May 09, 2020. In PCR, MgCl2 is an essential cofactor that enhances the activity of Taq DNA polymerase, which in turn increases the amplification rate of DNA. It is important to note, however, that higher concentrations of MgCl2 can result in decreased specificity.

Q. What is the purpose of PCR master mix?

The PCR Master Mix is designed for routine endpoint PCR for DNA amplicons in the range of 0.2–2kb. PCR Master Mix allows you to set up your reactions in less than a minute—just add template and primers. Optimized conditions enable amplification of as few as 2 copies of target template.

What is master mix used for?

The master mix enables researchers to set up controls and test different concentrations of their target DNA or RNA templates without having to individually add precise amounts of enzymes, buffers, cofactor (usually MgCl2), water and dNTP to each reaction tube or plate well.

What is Green Master Mix?

Description: GoTaq® Green Master Mix(a,b) is a premixed ready-to-use solution containing bacterially derived Taq DNA polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR.

Q. What is dream Taq?

DreamTaq DNA Polymerase is an enhanced Taq DNA polymerase optimized for all standard PCR applications. It ensures higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase. It is capable of robust amplification of up to 6 kb from genomic DNA and up to 20 kb from viral DNA.

Applied Biosystems® SYBR® Green PCR Master Mix combines SYBR® Green I dye, AmpliTaq Gold® DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial.

Q. What is the composition of GoTaq green master mix?

Description: GoTaq® Green Master Mix(a,b) is a premixed ready-to-use solution containing bacterially derived Taq DNA polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR.

Q. What is included in the GoTaq® qPCR master mix?

The GoTaq® qPCR Master Mix is provided as a ready-to-use, stabilized 2X formulation and contains GoTaq® Hot Start Polymerase, MgCl 2, dNTPs, BRYT Green® Dye and a proprietary reaction buffer. This master mix contains a low level of carboxy-X-rhodamine (CXR); a separate tube of CXR reference dye is included.

Q. How long does it take to set up a GoTaq master mix reaction?

Reactions assembled with the GoTaq® Master Mixes have sufficient density for direct loading onto agarose gels. Reactions can be set up in less than a minute; simply add your template, water and primers and go! GoTaq® Green and Colorless Master Mix reaction size is based on a 50µl reaction volume.

Q. What is in a 2x PCR master mix?

PCR Master Mix is a ready-to-use 2X solution containing Taq DNA polymerase, dNTPs, MgCl 2 and reaction buffers at optimal concentrations for efficient PCR amplification of DNA templates. The PCR Master Mix is designed for routine endpoint PCR for DNA amplicons in the range of 0.2–2kb.

Randomly suggested related videos:
PowerTrack™ SYBR Green Master Mix|Easy to use &flexible gene expression master mix for real-time PCR

PowerTrack™ SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR (qPCR). Pipette with confidence with the Applied …

No Comments

Leave a Reply

Your email address will not be published. Required fields are marked *